UNIVERSITY OF OXFORD
Department of Plant Sciences – 2011/2012
Research Topics and Supervisors willing to supervise Chemistry
Part II students.

Professor R.G. Ratcliffe (Plant Sciences) george.ratcliffe@plants.ox.ac.uk

Development of new NMR methods with applications in plant biology, including steady state, stable isotope labelling experiments for metabolic flux analysis, and protocols for testing metabolic hypotheses in isolated plant mitochondria. An interesting project for a Part II chemist would be to develop a combined 13C and 31P NMR approach for the analysis of metabolic pathways that are not traditionally associated with the mitochondrion.

1. R.G. Ratcliffe and Y. Shachar-Hill (2006) Measuring multiple fluxes through plant metabolic networks. The Plant Journal 45: 490-511.
2. S.K. Masakapalli, P. Le Lay, J.E. Huddleston, N.L. Pollock, N.J. Kruger and
3. R.G. Ratcliffe (2010) Subcellular flux analysis of central metabolism in a heterotrophic Arabidopsis cell suspension using steady-state stable isotope labeling. Plant Physiology 152: 602-619.
4. T.C.R. Williams, M.G. Poolman, A.J.M. Howden, M. Schwarzlander, D.A. Fell, R.G. Ratcliffe and L.J. Sweetlove (2010) A genome-scale metabolic model accurately predicts fluxes in central carbon metabolism under stress conditions. Plant Physiology 154: 311-323.