Biomolecules MS Service: Sample submission and data analysis
Sample submission
To submit a sample to the biological mass spectrometry service you need to fill out a sample submission form proteins, peptides and oligonucleotides which can be found at: \\Chem-ds5\massstore\Submission Forms for Mass Spec Service. Submit this in the ‘Biological Mass Spectrometry ‘In’ tray’ which is located in the basement mass spec room, next to the fridge freezer, next to the mass spec office (see photograph). Please ask a member of the MS staff is you cannot locate these trays.
Your sample vials should be placed in the "Biomolecular samples" submission box which can be found in the fridge freezer on the lab bench next to the mass spectrometry office in the basement.
 
Data Analysis and return
Protein and peptide samples will be analysed using LC/MS under denaturing conditions with a standard linear gradient from water to acetonitrile in 0.1% formic acid using C-4 or C-18 reversed phase chromatography.
Oligonucleotides will be run using an ion-pairing separation with a C18 column.
Unless specific instructions are given on the sample submission form the standard analysis will provide a TIC LC/MS chromatogram from the analysis along with a deconveluted mass spectrum (Maxent 1, Waters Corp) for major chromatographic peaks. These will be returned to the ‘out’ section of the ‘Biological Mass Spectrometry Services Tray’ by the 5pm on Monday the following week. Due to the complex nature of both experiments, data analysis and interpretation of results for this type of analysis it is encouraged you discuss your results with a member of the MS staff. Please email Lingzhi Gong (Lingzhi.gong@chem.ox.ac.uk) to arrange a time on the following Tue to discuss your results.
Data files: If the data files are required they will be available for one week only. Due to their size please see Lingzhi Gong if you wish to take copies away with you.
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