Modern NMR Spectroscopy for the Research Chemist
Dr Tim Claridge
Fridays 11-12 am from 19th October 2012 (week 2 onwards)
(No lecture on November 16th)
Wolfson Seminar Room, CRL
This eight-lecture Graduate level course is designed to introduce the modern NMR techniques most commonly used in synthetic chemistry research laboratories and builds on the introductory material found in most undergraduate chemistry courses. It does not introduce the basics of NMR spectroscopy and assumes a basic working knowledge of 1D organic NMR.
It is presented at a level appropriate for Part II, D.Phil and post-doctoral scientists who use NMR spectroscopy as a research tool. It will use non-mathematical descriptions of experiments aimed at highlighting the advantages and limitations of the methods in a practical context, covering basic 1D methods and more sophisticated 2D techniques.
There are no formal handouts for the course, but a pdf copy of slides will be posted here before each lecture and may be used for note taking (Oxford only)
Outline:
Lecture 1: Introducing Modern NMR- historical context, basic elements of NMR, sensitivity, relaxation, repetition, quantification [download pdf]
Lecture 2: 1D NMR methods- optimising 1D data, decoupling, sensitivity enhancement (INEPT), spectrum editing (DEPT and DEPTQ) [download pdf]
Lecture 3: 2D NMR methods I- homonuclear correlation spectroscopy (COSY and variants), total correlation spectroscopy (TOCSY), carbon-carbon correlations (INADEQUATE) [download pdf]
Lecture 4: 2D NMR methods II- heteronuclear correlation spectroscopy- one-bond correlations (HMQC and HSQC), editing HSQC, long-range correlations (HMBC and H2BC), hybrid methods (HSQC-TOCSY) [download pdf]
Lecture 5: the nuclear Overhauser effect- equilibrium vs transient NOEs, 1D and 2D nOe spectroscopy (NOESY), rotating-frame nOes (ROESY), heteronuclear nOes (HOESY) [download pdf]
Lecture 6: Methods for studying molecular interactions- diffusion NMR spectroscopy (DOSY), protein-small molecule (ligand) interactions (STD, WaterLOGSY etc), [download pdf]
Lecture 7: NMR Pulse Sequences: An introduction to NMR pulse sequences: the vector model, spin-echoes, generating 2D spectra, inversion recovery, INEPT and HSQC. [download pdf]
Lecture 8: How NMR works: The spectrometer, signal selection (phase cycling and pulsed field gradients), selective excitation, solvent suppression, inside the spectrometer. [download pdf]
Bibliography:
High-resolution NMR techniques in Organic Chemistry (2nd Edn), TDW Claridge, Elsevier, 2009.
Spectrometric Identification of Organic Compounds (7th Edn), RM Silverstein, FX Webster and DJ Kiemle, Wiley, 2005.