Membrane protein stoichiometry determined from the step-wise photobleaching of dye-labelled subunits

Symplectic ID: 
83383
Source: 
Scopus
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Monday, 14 September, 2026 - 14:00
DOI: 
10.1002/cbic.200600474
Publication Date: 
Monday, 18 June, 2007
First Page: 
994
Last Page: 
999
Authors: 
Das, SK
Darshi, M
Cheley, S
Wallace, MI
Bayley, H
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Abstract: 
Body count.In a generally applicable approach, the number of subunits in fluorescently-labelled protein complexes has been determined by counting photobleaching steps from individual molecules (see figure). The distribution of steps in the pore-forming toxins α-haemolysin and leukocidin indicate seven subunits for α-hemolysin, and four LukF and four LukS subunits for leukocidin. (Graph Presented). © 2007 Wiley-VCH Verlag GmbH&Co. KGaA.
ISSN: 
1439-4227
Journal Title: 
Chembiochem
eISSN: 
1439-7633
Volume: 
8
Issue: 
9
ID at Source: 
2-s2.0-34547510914
Publication Status: 
Published
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ptch0030