Aminoalkyl and alkyne-tagged PNA HyBeacons have been synthesized, labeled with fluorescein via conventional amide bond or triazole formation (click chemistry) and used to detect single nucleotide polymorphisms (SNPs) implicated in statin-induced myopathy. The PNA HyBeacons gave much better mismatch/mutant discrimination than conventional DNA HyBeacons but smaller fluorescence changes on melting.
Keywords:
Humans
,Muscular Diseases
,Oligonucleotide Probes
,Peptide Nucleic Acids
,Hydroxymethylglutaryl-CoA Reductase Inhibitors
,Fluorescent Dyes
,DNA Mutational Analysis
,Polymorphism, Single Nucleotide
,Transition Temperature
,Mass Spectrometry
,Click Chemistry
,Genotyping Techniques
